Why is this research important for Alberta ag?
Clubroot disease of canola caused by plasmodiophora brassicae (Pb) continues to spread across the prairies, posing a major immediate threat to canola production and long-term effect on soil health. Genetic resistance is the best method to control clubroot, but rapid emergence of "new" virulent pathotypes necessitates the search for new sources of resistance.
Researchers have identified a Brassica napus (Bn) line (SRC-37) with robust resistance to the Pb pathotype 3 (Pb3). Preliminary data shows the resistance in SRC-37 is dominant and monogenic. Researchers have conducted extensive RNA-sequencing on SRC-37 and the susceptible Bn cv. Topas, enabling identification of differentially expressed, known defense and novel genes.
This research will conduct fine mapping of the resistance (R) gene (PbR1) in SRC-37, develop markers for marker-assisted breeding, and introgress PbR1 to the spring type Topas. In addition, researchers will identify novel Bn defense genes effective against clubroot by functional analyses of differentially expressed genes revealed in RNA-Seq data.
What benefits can producers expect from this research?
Identifying such novel defense response regulators and effectors will lead to candidate genes for development of broad-spectrum resistance to Pb as a sustainable solution the the rapidly evolving clubroot pathogen.
How will these research findings reach producers on-farm?
AAFC will provide results to canola farmers. Data will be shared with the canola growers' organizations and farmers through presentations at regional meetings, publication in farmer-oriented magazines, newsletters and participation in canola field days.